Autoregulation of Lantibiotic Bovicin HJ50 Biosynthesis by the BovK-BovR Two-Component Signal Transduction System in Streptococcus bovis HJ50
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منابع مشابه
Autoregulation of lantibiotic bovicin HJ50 biosynthesis by the BovK-BovR two-component signal transduction system in Streptococcus bovis HJ50.
Streptococcus bovis HJ50 produces a lacticin 481-like 33-amino-acid-residue lantibiotic, designated bovicin HJ50. bovK-bovR in the bovicin HJ50 biosynthetic gene cluster is predicted to be a two-component signal transduction system involved in sensing signals and regulating gene expression. Disruption of bovK or bovR resulted in the abrogation of bovicin HJ50 production, suggesting both genes p...
متن کاملBovicin HJ50, a novel lantibiotic produced by Streptococcus bovis HJ50.
A bacteriocin-producing strain was isolated from raw milk and named Streptococcus bovis HJ50. Like most bacteriocins produced by lactic acid bacteria, bovicin HJ50 showed a narrow range of inhibiting activity. It was sensitive to trypsin, subtilisin and proteinase K. Bovicin HJ50 was extracted by n-propanol and purified by SP Sepharose Fast Flow, followed by Phenyl Superose and Sephadex G-50. T...
متن کاملCharacteristics of the bovicin HJ50 gene cluster in Streptococcus bovis HJ50.
Bovicin HJ50 is a new lantibiotic containing a disulfide bridge produced by Streptococcus bovis HJ50; its encoding gene bovA was reported in our previous publication. To identify other genes involved in bovicin HJ50 production, DNA fragments flanking bovA were cloned and sequenced. The bovicin HJ50 biosynthesis gene locus was encoded by a 9.9 kb region of chromosomal DNA and consisted of at lea...
متن کاملDissection of the bridging pattern of bovicin HJ50, a lantibiotic containing a characteristic disulfide bridge.
Bovicin HJ50, a lantibiotic produced by Streptococcus bovis HJ50, is featured by the presence of a disulfide bridge. This study described a simplified in vitro synthetic strategy for producing bovicin HJ50 totally based on Escherichia coli expression system. In this strategy termed as Semi-in vitro biosynthesis (SIVB), prepeptide BovA and modification enzyme BovM were co-expressed to generate p...
متن کاملBovicin HJ50-Like Lantibiotics, a Novel Subgroup of Lantibiotics Featured by an Indispensable Disulfide Bridge
Lantibiotics are ribosomally-synthesized and posttranslationally modified peptides with potent antimicrobial activities. Discovery of novel lantibiotics has been greatly accelerated with the soaring release of genomic information of microorganisms. As a unique class II lantibiotic, bovicin HJ50 is produced by Streptococcus bovis HJ50 and contains one rare disulfide bridge. By using its precurso...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2011
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.01278-10